Click on image to view larger version.
Figure 1. The effect of TEGDMA on the induction of apoptosis and necrosis in human primary pulp cells. Cell cultures were incubated with annexin V-FITC (apoptotic cells) and PI (necrotic cells) and analyzed by flow cytometry. The representative dual-parameter fluorescence histograms were derived from cell cultures exposed to various TEGDMA concentrations for 24 hrs. The numbers indicate the percentages of the viable (annexin V-; PI-) cell population (lower left quadrant), the apoptotic (annexin V+) cell population (lower right quadrant), and the necrotic cell populations in the upper right (annexin V+; PI+) and the upper left quadrants (annexin V-; PI +) in one typical experiment.
