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Figure 3. Immunohistochemical staining at week 4. (a) Anti-CD3 staining of the control group. (b) Anti-CD3 staining of the 20 mg/kg/day group. (c) Anti-CD25 staining of the control group. (d) Anti-CD25 staining of the 20 mg/kg/day group. Scale bars = 100 µm. (e) CD3+ cell-counts in the lesions of each group (n = 6). (f) CD25+ cell-counts in the lesions of each group (n = 6). (g) ED1+ cell-counts in the lesions of each group (n = 6). (h) TRAP staining of the control group. (i) TRAP staining of the 20 mg/kg/day group. Values are the means ± SD. *A value of p < 0.01 was determined as statistically different in comparison with the controls.
