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Figure 1. Apoptotic gene expression in wild-type (WT) and TNFR1–/–R2–/– mice measured by the RNase Protection Assay. Total RNA extracted from tissue obtained 24 hrs following inoculation of LPS or vehicle alone was subjected to RPA. (A) The PhosphoImages of gene expression in wild-type (+) and TNFR1–/–R2–/– (-) mice. (B) Densitometric analysis of apoptotic gene expression in wild-type and TNFR1–/–R2–/– mice. The densitometric value of each band was normalized by the value for GAPDH in the same lane. The percentage change values after LPS injection vs. vehicle injection are displayed. Each value represents the mean of 3 RPA ± SEM.
