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Figure 4. Light micrographs of 10-µm-thick sagittal serial sections of the posterior portion that faces the upper compartment of non-forced rat TMJs (non-TMJ), with the ISNT (a,b) and TUNEL (c,d) methods for staining DNA strand breaks. The sections were pre-treated without proteinase K (PK-) (a,c), or with PK (PK+) (b,d). (a,b) In the section that was pre-treated without PK (a) or with PK (b), there is no reactivity for ISNT in the synovial lining cells of the synovitis-induced TMJs with G0 synovitis. F: synovial fold. UC upper joint compartment. (c,d) In the TUNEL-stained sections that were treated without PK (c) or with PK (d), the synovial lining cells of the synovitis-induced TMJs with G0 synovitis are negative for TUNEL. Eosin staining. Original magnification: x400. Bar = 25 µm.
