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Figure 4. Lipopolysaccharide-receptor interactions: Cementoblasts (OCCM-30) were cultured in DMEM (5% FBS) with ascorbic acid (AA) (50 µg/mL) (control-CN), plus P-LPS (100 ng/mL), or P-LPS plus Antibody (against TLR-4/MD-2 complex-MTS510) (20 µg/mL). (A) RNA was extracted after 24 hrs, and expression of bone sialoprotein (BSP), osteocalcin (OCN), collagen I (Col I), and osteopontin (OPN) was assessed. Values were normalized to 18S, and bars represent mean ± SD from two separate experiments, with similar results (n = 2). Note that antibody treatment partially blocked the effect of P-LPS on OCN/OPN/Col I mRNA levels. (B) Real-time PCR for RANKL/OPG mRNA expressions. Results illustrate that antibody also partially blocked the effect of P-LPS on RANKL/OPG. Antibody treatment resulted in a significant reduction of the effect of P-LPS on OPG, whereas its effect on RANKL expression was not statistically significant. Bars represent mean ± SD from two separate experiments (n = 2). *P < 0.001 and **P < 0.05 vs. control.
