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Figure 3. The autogenous transplantation of the pellet culture on the canine amputated pulp. (A) Canine pulp cells transduced with adenovirus lacZ before the pellet culture began and stained by ß-galactosidase on day 14. (B) The implanted pellet on the amputated pulp showing lacZ transgene expression on day 28. The amputated site (arrow). (C) The amputated pulp without transplantation of the pulp cell pellet. Note no osteodentin matrix formation after 4 wks. (D) The in vivo transplantation of the pellet without rhBMP2. (E) The transplantation with rhBMP2. Note the formation of the thicker osteodentin matrix (OD) beneath the amputated site (arrows) in response to cell therapy with rhBMP2 compared with cultures without rhBMP2. (F–H) Higher magnification of osteodentin. Fewer cells and more homogenous matrix surrounding cells in rhBMP2-supplemented implantation (G) compared with that without rhBMP2 (F). Note the dentinal tubes (arrows) in the matrix (H).
