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Figure 2. Histological appearance of bone apposition. (A) At 2 wks, bone is deposited on the bony wall of the tissue chamber and on the implant surface. Both layers are connected by a scaffold of tiny trabeculae. Woven bone is characterized by the intense staining of the mineralized matrix and the numerous osteocytes located in large lacunae (undecalcified ground section, surface-stained with toluidine blue and basic fuchsin; bar = 500 µm). (B) At 4 wks, the volume density of this scaffold has increased both by the formation of new trabeculae and by deposition of more mature, parallel-fibered bone onto the primary scaffold. Woven bone is mainly recognized by the numerous large osteocytic lacunae (bright). The gap between bone and implant surface is an artifact (bar = 500 µm). (C) At 8 wks, growth and reinforcement result in a further increase in bone density and an almost perfect coating of the implant surface with bone. Remodeling has started, replacing the primary bone by secondary osteons (arrows; bar = 500 µm).
