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Figure 1. Cultured 4-dpn rat tooth bud cells. After 6 days in culture, tooth bud cells appeared heterogeneous, containing both rounded epithelial and fibrotic mesenchymal cell types (A,B). Cytokeratin-14-positive positive-control oral epithelial cells and dental epithelial cells in mixed cell culture appeared brightly fluorescent (arrows, C and D, respectively). Dental cells seeded onto PGA and PLGA scaffolds (E and F, respectively).

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