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Figure 2. Adhesion of biotinylated A. naeslundii parent and fimbriae-deficient mutant strains to: (A) nitrocellulose membranes spotted with 10 ng purified PRP-1 or PRG and 1 µg MG2 or asialo-MG2; or (B) blots of parotid (P) and SM-SL (S) secretions separated by SDS-PAGE. The presence or absence of type 1 and type 2 fimbriae on each strain is indicated in parentheses. Adherent bacteria were detected with avidin-D alkaline phosphatase. Adhesion assays were also performed in the presence of 2 mM EGTA where indicated. Blots remained untreated (UT) or were sialidase-treated (SIAL) prior to the addition of bacteria.
