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Figure 1. Detection of salivary proteins and glycoproteins following separation of parotid (P) and SM-SL (S) saliva by SDS-PAGE. (A) Gels were silver-stained or transferred to nitrocellulose prior to the labeling of amino and sulfhydryl groups. Glycosylated proteins were labeled and detected on blots following oxidation of sialic acids with 1 mM sodium periodate or total carbohydrate with 10 mM sodium periodate (IO4–). (B) Individual blots were overlaid with biotinylated lectins, and bound lectin was detected with avidin-D alkaline phosphatase. The lectin probes used were LFA for terminal sialic acid, PNA for terminal Galß1-3GalNAc, LTA for L-fucose, and ConA for glucose and mannose. Blots remained untreated (UT) or were sialidase-treated (SIAL) prior to incubation with lectins. The locations of purified MG1, MG2, PRG, 
-amylase (AMY), and PRP-1 are indicated.
