Click on image to view larger version.

Figure 2. Immunostaining showed Ihh expression in the proliferative layer (arrowhead) and the chondroblast layer (arrow) in the mandibular condylar cartilage (A) and tibia growth plate (B) of 42-day-old rats during natural growth. A significantly higher level of Ihh expression was detected in the proliferative layer of condylar cartilage during mandibular advancement (D, 7 days after experiment) than that of during natural growth (C, 42-day-old rats). Scale bars: 400 µm for A; 50 µm for B, C, and D.