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Figure 1. Increased expression of HIV receptors in gingival inflammation. Representative serial sections of (A) healthy (n = 8) and (B) inflamed gingiva (n = 12) obtained from surgical crown lengthenings and during periodontal surgery for chronic periodontitis, respectively, were subjected to single immunoenzyme labeling for DC-SIGN (1), mannose receptor (MR [2]), and Langerin (3) and also stained for H&E (4). 20x fields were scanned by image analysis, as described in MATERIALS & METHODS, and final magnification shown (optical and digital) is approximately 250x. Positively stained cells are brown, some of which are indicated by arrows. Shown are the mean numbers (± SE) of (C) DC-SIGN+, (E) MR+, and (G) Langerin+ cells in healthy and inflamed gingiva. *Statistically significant difference (p < 0.05, Student’s t test). (D,F,H) Linear regression analysis was performed on the number of inflammatory cells (H&E-positive/20x field in lamina propria) (Y-axes) and the number of (D) DC-SIGN+, (F) MR+, and (H) Langerin+ cells (x-axis) per 20x field.
