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Figure 1. Expression of WNT family members in oral squamous carcinoma cell lines. (A) A panel of WNT members amplified by RT-PCR is listed in the Table . Normal keratinocytes (NKC1 and NKC2) and fibroblasts (GF12) isolated from gingiva were included. (B) Representative data of RT-PCR for WNT3, WNT6, and GAPDH as an internal control (lane 1, OSC19; lane 2, HSC2; lane 3, TSU; lane 4, HOC313; lane 5, Ca9.22; lane 6, KOSC3; lane 7, Ho1u1; lane 8, SCCTF; lane 9, SCCKN; lane 10, HSC3; lane 11, KOSC2). RNA sample isolated from normal gingival keratinocytes (lane 12, NKC1; lane 13, NKC2) and GF12 normal gingival fibroblasts (lane 14) were also applied. PCR reactions without reverse transcription of OSC19 cells were also performed as a negative control (-RT).
