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Figure 3. Occurrence of mRNA of eNOS in 300 bp (upper part) and iNOS in 340 bp (lower part) by RT-PCR in human dental pulp in the healthy tissue (A), hyperemic (B), and irreversible pulpitis (C). The standard band is on the right lane (S 488 bp). The Fig. clearly shows the presence of eNOS 300 bp in all 3 groups, with the highest level in the hyperemic group. The presence of iNOS 340 bp in the healthy pulp is not detectable; however, a marked increase of iNOS 340 bp was found in hyperemic and irreversible pulpitis groups. 18S (488 bp) is the internal standard. Data are expressed as means ± SD of 5 pulps in each pulp condition. Significant differences of integrated optical density (IOD) of eNOS and also iNOS mRNA, indicated in Table 2 (Appendix), were found in all 3 groups (*p < 0.05, A vs. B; *p < 0.05, B vs. C; *p < 0.05, A vs. C in both cases).
