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Figure 2. Development of CRF expression in the rat PVN during the 240-minute period after 60 min of restraint. (A) Photomicrographs of the right PVN from a control rat (control) and those with different times of 0, 60, 120, 180, and 240 min after 60 min of restraint. Scale bar = 500 µm. (B) The average numbers (means ± SEM, n = 6 in all groups) of CRF-positive neurons detected on the left (open columns) and right (filled columns) sides of the PVN reached the maximal level at 180 min post-restraint. *p < 0.05 (ANOVA/Fisher’s PLSD) with respect to the controls.
