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Figure 1. Expression of CRF-positive neurons in the rat PVN. (A) A schematic drawing of a coronal section of the rat brain through the level of the hypothalamus. A square in the broken line indicates the approximate area of the right PVN shown in photomicrographs (B) and (C). CRF-expressing neurons detected in a non-stress control rat (B) and those induced after 60 min of restraint stress and 180 min recovery (C). (D) Schematic drawings depicting the serial sections of the right hypothalamus of the same rat shown in panel (C) between -1.60 and -2.10 mm from the bregma. Stress-induced CRF-positive neurons were mainly localized within the PVN indicated by a broken line.
