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Figure 3. Numbers of adherent MC3T3-E1 and L 929 cells on the specimens after 1 wk of cultivation. The number of adherent cells for each cell line on Ti-Cl was significantly higher than those on control specimens. Adherent cells were incubated with 100 µL/mL of assay reagent solution for 1 hr, and then the absorbance at 450 nm was measured. The number of adherent cells was calculated from the activity of the original cell suspension. The results are expressed as the mean ± SD of 6 specimens (n = 6). The findings were analyzed statistically by ANOVA. *Significant differences from respective control values were considered to exist when p < 0.01.
