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Figure 2. TEM photographs of a positive control (A), an ultrastructural image (B), and immunogold staining images (C,D) of the PFZ. (A) Immunolabeled gold particles are observed along the fimbriae of a P. gingivalis cell, reacted with the anti-fimbriae serum. (B) Small numbers of bacterial cells, including a filamentous organism (arrow), attached to the DC covering acellular cementum (C), and aggregated with other cells. (C,D) Gold particles (arrows) can be observed along the fimbriae of the cells, in close contact with the DC in the PFZ. DC in panels B, C, and D: dental cuticle. Bar = 0.1 µm (A); bar = 0.5 µm (B-D).