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Figure 2. Immunostaining images for PCNA (A-C) and desmin (D) in the somites 2, 3 (A), 4, 5 (B), and 3 (C,D) of the E9 mouse embryo. The diagrammatic representations show sagittal sections of tongues at E11 (E) and E13 ~ birth (F), viewed from the buccal side. The dotted rectangles indicate the regions in which proliferating cell nuclear antigen (PCNA)-labeling indices were obtained. Immunostaining images for PCNA (G,H,K-N) and fast myosin heavy-chain (I,O-R) in the whole portion of the E11 tongue (G,I), and in the anterior (K,L,O,P), middle (M,Q), and posterior (N,R) regions of E13 tongues. A control staining image of a whole portion of an E11 tongue treated with normal mouse IgG instead of the antibodies (J). H, L, and P are higher-magnification images of the regions indicated by the rectangles in G, K, and O, respectively. White lines in L contour the areas which lack the PCNA-positive cell nuclei.
