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Figure 3. Immunohistochemical analysis of OPG. Immunofluorescence analyses of serial sections were performed with anti-OPG antibodies. Images (a) to (c) are photographs of the palatal sides of first molars. Fluorescence image and phase-contrast image were superimposed on the software (Adobe Photoshop 5.0, San Jose, CA, USA). (a) Photograph of the tooth-movement group. Representative photographs of 6 sections are shown. (b) Photograph of the OPG-transfection group. Representative photographs of 5 sections are shown. (c) Photograph of the mock group. Representative photographs of 4 sections are shown. Bar = 100 µm. Bu, buccal side. Pa, palatal side. (d) Combined photograph of the OPG-transfection group. Series of the fluorescent photographs taken under the same exposure conditions were combined. Strong OPG expression was observed at M1. (e) Photograph of the OPG-transfection group stained with hematoxylin-eosin. About the same field as shown in Fig. 3d is presented. Bar = 1 mm.
