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Figure 3. 3-Nitrotyrosine immunoreactivity increases in hamster cheek pouch tissues with tobacco-related-compound application. (A) Tissue treated with DMSO/mineral oil; (B) smokeless tobacco extract; (C) nicotine; (D) NNN; and (E) NNK. Control epithelium shows 3-nitrotyrosine immunoreactivity confined to basal cells (arrows). All tobacco-related-compound treatments resulted in full-thickness 3-nitrotyrosine immunoreactivity. Scale bar represents 70 µm. (F) Mean gray level values ± SD of 3-nitrotyrosine intensity from 15 50 x 50 pixel regions of interest in 2 to 5 animals (* p < 0.005 compared with control).
