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Figure 4. Effects of PDTC on ROS production, apoptosis, and I
B
degradation induced by HEMA. Cells were pre-treated with 10 µM PDTC for 30 min. (A) The cells were loaded with DCFH-DA and further treated with 10 mM HEMA and PDTC for 30 min. DCF fluorescence was analyzed by flow cytometry. Results represent the means ± SEM (n = 4). (B) Apoptotic and necrotic cells were detected after 10 mM HEMA and PDTC treatments for 24 hrs. Results represent means ± SEM (n = 3). *Values are significantly different from untreated controls (one-way ANOVA, followed by the Bonferroni post hoc test, p < 0.05). (C) IB degradation was evaluated after 10 mM HEMA and PDTC treatment for 180 min. Western blot is representative of 2 independent experiments. (D) HEMA-induced apoptosis in MEF wild-type vs. p65–/– cells. MEF and p65–/– cells were treated with 8 mM HEMA for 24 hrs. Apoptotic (lower right quadrant) and necrotic (left and right upper quadrants) cells were then detected by flow cytometry. The dot plot is representative of 3 independent experiments.
