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Figure 2. Generation of ROS by HEMA in human fibroblasts. Suspended cells were incubated with 10 µM DCFH-DA for 15 min at 37°C. (A) The indicated concentrations of HEMA were added to the cells and incubated for 30 min at 37°C. (B) From 0 to 10 mM HEMA was added to the cells and incubated for 15, 30, and 60 min at 37°C. DCF fluorescence was measured by means of a flow cytometer with an FL-1 filter. Results represent the means ± SEM (n = 5). *Values are significantly different from untreated controls (one-way ANOVA followed by the Bonferroni post hoc test, p < 0.05).
