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Figure 3. FIV(lacZ) and FIV(
'lac) injections (5 x 106 infectious particles) in the right TMJ of mice resulted in successful infection of primary sensory neurons located in the ispilateral trigeminal ganglion. The animals’ left-side TMJ was not treated. (A) The presence of backbone FIV DNA in the right trigeminal ganglia ipsilateral to FIV injections was detected by a 444-bp DNA band in lanes 1 and 3, utilizing the "FIV" primers (as depicted in panel A), suggesting successful transduction of the trigeminal sensory neurons by FIV vectors. Lanes 2 and 4 do not display any viral DNA, since they represent left-side ganglia. (B) The inactive form of the ß-galactosidase gene in transduced neurons was detected by the absence of the 1.7-kb DNA band (lane 1) compared with the wild-type gene (lane 3). Lanes 2 and 4 do not display any viral DNA, since they represent left-side ganglia. (C) The successful extraction of genomic DNA from left and right ganglia was confirmed by PCR utilizing primers designed for the murine housekeeping gene G3PDH (385 bp).
