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Figure 2. Immunoblotting of human pellicle, purified lysozyme, lactoferrin, carbonic anhydrase II, and PRP1 with specific antisera. Purified proteins (0.25 µg) or pellicle (40 µg) was subjected to Tris-tricine (Panels A-C) or Ornstein-Davis (Panel D) PAGE and blotted onto PVDF membranes. Panel A, blot was probed with 1:100 diluted anti-lysozyme anti-serum. Panel B, blot was probed with 1:250 diluted anti-lactoferrin anti-serum. Panel C, blots were probed with 1:1000 diluted anti-carbonic anhydrase II anti-serum. Panel D, blot was probed with 1:2000 diluted anti-PRP1 anti-serum. Upper arrow, large PRPs (PRP1, 2 and PIFs); lower arrow, small PRPs (PRP3, 4 and PIFf).
