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Figure 1. Reactivities of mouse anti-pellicle antiserum against purified protein standards in ELISA. Pre- and post-immune sera were collected from 2 mice before the first injection with pellicle and ten days after the 4th boost with pellicle proteins, respectively. Microtiter plates were coated with pellicle proteins (10 µg/mL) or a series of purified protein standards (2 µg/mL) and subsequently incubated with 1:1000 diluted pre-immune serum (white bars) or 1:1000 diluted post-immune serum (black bars). Each bar represents the mean value ± SD of one experiment performed in triplicate. Panel A, mouse #1; Panel B, mouse #2.
