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Figure 1. Amounts of hyaluronan (HA) in synovial membrane fibroblast cultures treated with and without transforming growth factor beta 1 (TGF-ß1) measured by means of high-performance liquid chromatography and differential refractometer. (A) < 300 kDa HA in the conditioned medium (mean ± SD, n = 3). (B) < 300 kDa HA in the cell layer (mean ± SD, n = 3). (C) 300 ~ 1900 kDa HA in the conditioned medium (mean ± SD, n = 3). (D) 300 ~ 1900 kDa HA in the cell layer (mean ± SD, n = 3). (E) > 1900 kDa HA in the conditioned medium (mean ± SD, n = 3). (F) > 1900 kDa HA in the cell layer (mean ± SD, n = 3). The amount of HA with TGF-ß1 treatment for 12 to 72 hrs was compared with the non-treatment control by Student’s t test. *p < 0.05, **p < 0.01.
