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Figure 2. Degradation of dhvar1 by S. mutans analyzed by MALDI-TOF. A suspension of S. mutans (6.4 x 108 cells/mL PBS) was incubated with 200 µg/mL dhvar1 for 5 hrs at 37°C. Subsequently, cells were removed by centrifugation, proteinases were inactivated by being heated for 5 min at 100°C, and samples were analyzed by MALDI-TOF. The original peptide (molecular mass 1855) was degraded into two fragments with molecular masses of 821 and 1054, corresponding to the fragments Lys1-Glu6 and Leu7-Tyr14, respectively. Potassium and sodium adducts contribute to minor adjacent peaks.
