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Figure 3. Effects of extracellular ions on Hst 5 binding to C. albicans cells. C. albicans cells (106) were treated with FITC-Hst 5 (31 µM) for 1 hr (filled areas) and analyzed on a FACSCAN flow cytometer (Becton Dickinson). For pre-treatment experiments, cells were mixed with (A) KCl (50 mM), (B) MgCl2 (10 mM), or (C) CaCl2 (10 mM) for 20 min (thin black lines), after which FITC-Hst 5 (31 µM) was added and incubated for an additional 40 min. For post-treatment experiments, cells were first treated with FITC-Hst 5 (31 µM) for 20 min, after which KCl (50 mM), MgCl2 (10 mM), or CaCl2 (10 mM) was added for an additional 40 min (bold lines) (A, B, C, respectively).
