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Figure 4. Densitometric results from semi-quantitative RT-PCR analysis (mean ± standard deviation). The magnitude-dependent effects of tension (A) and compression (B) on mRNA expression of MMP-8 and MMP-13 are indicated. Open bars, MMP-8; solid bars, MMP-13 (A and B: n = 6). Time course changes in the relative expressions of MMP-8 (C) and MMP-13 (D) are indicated. Open bars, control non-compressed cultures; solid bars, compressed cultures at 0. 2kPa (C and D, n = 4). The effects of conditioned media from cultures compressed or not compressed for 1 and 3 days on the expression of MMP-8 (E) and MMP-13 (F) were measured at 1 and 3 days of culture. Open bars, control, noncompressed media; solid bars, compressed media (E and F, n = 3). CM, conditioned media. **P < 0.01, *P < 0.05.
