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Figure 2. Structure of the PDL-29 gene. (A) PDL-29, PET112 cDNA sequences, and their chromosomal localization. Nucleotides 1-1984 of PDL-29 and 28-2011 of PET112 completely match, whereas only PDL-29 contains the insertion sequence just before the poly (A)-tail. Nucleotides 1-28 of PET112 and 1-161 of PDL-29 are localized to exon 1, and nucleotides 1530-2180 of PDL-29 are localized to exon 15. Filled area in PDL-29: insertion sequence (197 bp) just before the poly (A)-tail. Filled area in PET112: 5' region of PET112 different from that of PDL-29. Full-length PDL-29 (2180 bases) was submitted to GenBank (Nov. 1, 1998) and has been assigned accession no. AB019410.
(B) Insertion sequence in the 3'-UTR of PDL-29. Nucleotides 1984-2180 of PDL-29 and the poly (A)-tail are displayed. Horizontal bars indicate putative cis-acting elements of known molecules, and similarities are indicated in percentages. GH, bovine growth hormone; TCR Vß2.2, human T-cell receptor Vß2.2; OC, rat osteocalcin; ML-MuMTV, mouse mammarytumor-virus long terminal repeat; GPT, mouse N-acetylglucosamine-l-phosphate transferase; LYZ, chicken lysozyme; and PAI-2, human plasminogen activator inhibitor type-2.
