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Figure 1. Unique gene expression in HPFs and HGFs. (A) Osteocalcin mRNA expression in HPFs and HGFs as revealed by PCR analysis. The arrowhead at the right indicates the position of the amplicon. Complementary DNA (10 ng) from each cell was amplified by PCR with human osteocalcin primers (5'-ATGAGAGCCCTCACACTCCTC-3' and 5'-CAGGGGATCCGGGTAGGGGAC-3'). The amplification was performed for 40 cycles, and the annealing temperature was 55°C. M: 100-bp ladder. (B) Messenger RNA expression of PDL-29 in HPFs and HGFs. Messenger RNA from HPFs and HGFs was hybridized with PDL-29 cDNA probe. For an internal control, the mRNAs were also hybridized with human ß-actin probe. The donor is #1 in Fig. 1C. (C) Densitometric analysis of signal intensity. Relative signal intensities for PDL-29 mRNA from three different donors are shown after normalization against the signal for ß-actin mRNA.
