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Figure 2. Immunoblot analysis with serum from representative male IL-10(-/-) 129/SvEv mice aged 7 mos (panels B and C) and age- and sex-matched IL-10(+/+) 129/SvEv mice (panels D and E). Panel A: Stained SDS-PAGE of bacterial crude extracts. On the left margin, the relative position of the molecular weight standards is marked. Each lane was loaded with 20 µg of protein corresponding to extracts of Porphyromonas gingivalis (lane 1), Bacteroides forsythus (lane 2), Bacteroides fragilis (lane 3), Bacteroides vulgatus (lane 4), Prevotella intermedia (lane 5), and partially purified P. gingivalis GroEL (HSP-60) (lane 6). Panels B, C [IL-10(-/-) 129/SvEv sera] and D, E [IL-10(+/+) 129/SvEv sera]: Western blots of the bacterial extracts under identical conditions; primary antibody (mouse sera) at 1:4000 dilution, secondary antibody at 1:5000 dilution; lanes as in panel A.
