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Figure 3. RT-PCR of TGF-ß1, LTBP-1, and fibromodulin. RT-PCR was performed on RNA isolated from normal tissues or wound samples at indicated times post-injury. To determine relative changes in mRNA levels during development, we corrected densitometry values for each gel to ß actin expression at each time and normalized them by setting the highest value to 1. (A–C) The results are depicted graphically as the mean ± SE; n = 3. Data were analyzed by two-way ANOVA and Bonferroni’s post-test. (D) A representative PCR is shown. NS = normal skin.
