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Figure 4. Cell-cycle analysis of viable pulp cells and macrophages. Flow cytometry profile of MDPC-23, OD-21, or macrophages exposed to partially polymerized SingleBond (light-cured for 10 sec) and stained with propidium iodide. (a) Percentage of viable cells at each phase of the cell cycle (G1, S, G2). Oblique line bars depict untreated control cells, and solid black bars depict cells exposed for 12 hrs to the adhesive resin (bg). Cell-cycle profiles of cells exposed for 12 hrs to partially polymerized SingleBond and untreated controls. Data represent mean values (± SD) of triplicate samples per condition and cell type, and each sample consisted of approximately 10,000 cells.