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Figure 1. Adhesive resin induces apoptosis of pulp cells and macrophages. Percentage of apoptotic mouse odontoblast-like cells (MDPC-23) (a), undifferentiated pulp cells (OD-21) (b), or macrophages (RAW 264.7) (c) after a 12- or 24-hour exposure to unpolymerized, partially polymerized (light-cured for 10 sec), or polymerized (light-cured for 40 sec) SingleBond, respectively. Apoptotic cells were identified as a sub-G1 population after being stained with propidium iodide and sorted by flow cytometry. Asterisk indicates statistical significance at p 
0.05, as compared with untreated controls (white bars). Data represent mean values (± SD) of triplicate samples per condition and cell type, and each sample consisted of approximately 10,000 cells.
