Figure 4. Synthetic lipid A induces Raf activation in the cot/tpl2-/- osteoblasts. Osteoblasts isolated from the wild-type and cot/tpl2-/- mice were treated with 1 µg/mL synthetic lipid A for the indicated times. Raf-1 phosphorylation was analyzed with phospho-specific anti-Raf-1 antibody. The same filter was re-probed with an anti-Raf-1 Ab to show consistent amounts of the whole Raf-1 kinase.

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