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Figure 2. Impairment of synthetic lipid-A-mediated RANKL mRNA up-regulation in osteoblasts from cot/tpl2-/- mice. Osteoblasts isolated from calvaria of the wild-type and cot/tpl2-/- mice were treated with indicated doses of synthetic lipid A. At 2 hrs after the treatment, total RNA was extracted, separated in a 1% agarose/formaldehyde gel, and transferred to a nylon membrane. The gene expression of RANKL was analyzed by hybridization with the use of a 32P-labeled specific RANKL cDNA probe. The 18S and 28S ribosomal RNA bands of the ethidium-bromide-stained gel are shown as a control for equal loading.
