Figure 1. Expression of cot/tpl2 in primary mouse osteoblasts. Osteoblasts from calvaria of ddy mice were treated with 1 µg/mL synthetic lipid A for the indicated times. Cell lysates were prepared, and the amount of the cot/tpl2 protein was determined by Western blot analysis with the use of anti-cot/tpl2 polyclonal antibody. Two forms of the cot/tpl2 protein were translated from the alternative initiation codons (Aoki et al., 1991; Miyoshi et al., 1991).

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