Click on image to view larger version.
Figure 2. Effects of acetylcholine and epinephrine on AQP5 levels in the apical plasma membrane of parotid tissues from senescent and young adult rats. (A) Parotid slices from 10-week-old (Young) or 115-week-old (Old) rats were incubated for 15 sec at 37°C in the absence (C) or presence (ACh) of 1 µM acetylcholine and 1 µM eserine or incubated for 1 min at 37°C in the absence (C) or presence (Epi) of 10 µM epinephrine. The apical plasma membrane fraction (5 µg of protein) was subjected to immunoblot analysis with anti-AQP5 antibody. (B) Immunoblots were subjected to densitometric analysis, and the amount of AQP5 was expressed as a percentage of the control cell values. Data are expressed as means ± SE of 6 independent experiments. **P < 0.01, ***P < 0.001 vs. the value of the control cells.
