Figure 1. Western blots for AQP5 in the homogenate and M₃-muscarinic receptors and Gq/₁₁ protein in basolateral membrane of parotid tissues. Parotid slices from 10-week-old (Young) or 115-week-old (Old) rats were incubated for 10 min at 37°C in the absence (1, 3, 5, 6) or presence (2, 4) of 10 µM SNI-2011. The homogenate (40 µg of protein) (A) and basolateral membrane (40 µg of protein) (B,C) of parotid glands from young adult (1, 2, 5) and senescent rats (3, 4, 6) were subjected to immunoblot analysis with antibodies (1:1500 dilution) to AQP5 (A), M₃-mAChR (B), and Gq/₁₁ (C).

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