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Figure 1. Panning procedure. Step 1. The target protein (20 µg of MG2) was plated for 1 hr. Step 2. The plate was blocked for 1 hr. Step 3. We added the pFlitrx library to the plate to allow for interaction of expressed dodecapeptides on E. coli flagellae with bound target protein for 1 hr. Step 4. E. coli (unbound) that did not interact with immobilized target protein were washed off. Step 5. Bound cells were eluted by mechanical agitation. Step 6. Eluted cells were grown overnight at 37°C. The panning procedure was repeated 11 times.
