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Figure 1. (A) Effect of dentin extracts on cell viability. Thioglycolate-elicited macrophages were treated with particulate (D-part) and non-particulate (D-n-part) dentin extracts (5 mg/mL) in the presence or absence of IFN-
(60 U/mL), LPS (500 ng/mL), IFN-/LPS, and L-NMMA (200 µM), and cell viability was determined by MTT reduction assay after 48 hrs. *Significantly different from respective control values at P < 0.05. (B) Effect of polymyxin B on nitric oxide production induced by dentin extracts. Peritoneal macrophages were stimulated with particulate dentin extract (D-part) (5 mg/mL) and LPS (500 ng/mL) pre-incubated or not with polymyxin B as described in MATERIALS & METHODS. *Significantly different from respective control values at P < 0.05. #Indicates a level of significance (P < 0.01) relative to polymyxin B-untreated extracts. Data represent the mean + SEM of triplicates.
