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Figure 3. Expression of bcl-2 family genes between PDLF and GF. Human PDLF and GF were cultured, and apoptosis was induced by serum deprivation as described in MATERIALS & METHODS. The mRNA levels of bcl-2 family members were measured by RNase protection assay for anti-apoptotic genes (A,B) and pro-apoptotic genes (C,D). Radioactive signals were quantified with a PhosphorImager equipped with IMAGEQUANT software (Molecular Dynamics). A GAPDH probe was used as an internal control. Mean ± SD, n = 3. *P < 0.05, **P < 0.01, t test, difference between PDLF and GF.
