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Figure 3. Induction of apoptosis and necrosis by different concentrations of TEGDMA after 24 hrs. FACS analysis after staining with Annexin V-FLUOS/PI. Three distinct cell distribution patterns are visible: normal, viable cells (lower left quadrant); apoptotic fibroblasts (lower right quadrant); and necrotic and/or "apoptotic necrotic" HGF (upper right quadrant). (A) Non-treated cells (negative controls). Most cells are ‘located’ in the lower left quadrant (viable cells). (B) Cells treated with 1 mM TEGDMA (similar to A). (C) Cells treated with 2.5 mM TEGDMA. A slight shift toward the area indicating "apoptotic" cells is noticeable. (D) Cells treated with 5 mM TEGDMA. (E) Cells incubated with 7.5 mM TEGDMA. (F) Cells incubated with 10 mM 5-fluorouracil (positive control); Annexin V-FLUOS/PI staining reveals the shift of the cells toward apoptosis. (D) and (E) clearly reveal a significant increase in the share of apoptotic cells as well as a moderate shift toward the upper right quadrant, which is indicative of necrotic and/or "apoptotic necrotic" cells. (A-F) Diagrams of one representative experiment each.
