Figure 2. Effects of eugenol on [Ca²⁺]_i in VR1-expressing HEK 293 cells. (A) Eugenol (1 mM) increased [Ca²⁺]_i in a capsaicin-responsive cell. Carbachol (CCh, 10 µM), as a positive control, evoked Ca transient through muscarinic receptors endogenously expressed on the cells. The eugenol-induced Ca transient was abolished by CZP (10 µM) (B) and RR (10 µM) (C).

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