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Figure 3. Amelogenin promotes both cell attachment and cell spreading. Using the cell adhesion assay described in MATERIALS & METHODS, we found MG63 cells to attach to and spread on plastic substrata coated with 100 µg/plate Emdogain® (Panel A), 100 µg/plate amelogenin (Panel B), 100 µg/plate fibronectin (Panel C), and control (heat-inactivated BSA only) (Panel D). Note that the rounded cells in Panels A-C were attached well enough to resist washing of the plate, while the rounded cells in the control (Panel D) would have been removed if the plate had been washed.
