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Figure 1. SDS-PAGE analysis of Emdogain® and recombinant porcine amelogenin. Samples of Emdogain® and recombinant porcine amelogenin were analyzed by SDS-PAGE on 15% (w/v) cross-linked polyacrylamide mini-slab gels under reducing conditions and stained with Coomassie Brilliant Blue R-250. The analysis showed that Emdogain® contains several protein species. The molecular masses were clearly detectable from less than 14.3 to over 77 kDa. A major protein band, corresponding to processed
20-kDa amelogenin, was clearly visible in the Emdogain® preparations. In comparison, the native, unprocessed recombinant porcine amelogenin migrated as a single band with a mass of
25 kDa. Mr, molecular-weight markers x 10-3, are indicated.