Figure 1. SDS-PAGE (10%) of pellicles formed on orthodontic brackets. Lane 1, Unstimulated whole saliva; Lane 2, experimental bracket pellicle (EBP) formed on metal brackets; Lane 3, EBP on polycrystalline alumina brackets; Lane 4, EBP on monocrystalline sapphire brackets; and Lane 5, EBP on plastic brackets. The gel was stained with 0.25% Coomassie brilliant blue. Immunodetection of these components identified, the 13- to 20-kd molecule as cystatins, the 30-kd molecule as acidic proline-rich proteins, the 55-kd molecule as 
-chain of sIgA, the 60-kd molecule as -amylase, and the 120-kd molecule as low-molecular-weight mucin.
