Figure 2. (A) Effect of 50 µg/mL OFE on osteocalcin, bone sialoprotein (BSP), and alkaline phosphatase (ALP) mRNA expression in cultures of ST2 cells. Total RNA of ST2 cells incubated with or without OFE was collected on days 1, 4, 7, 11, and 14. Each culture was incubated with 50 µM ascorbic acid, 10 mM ß-glycerophosphate, and 200 nM retinoic acid. (B) Nodule formation induced by OFE. OFE was added to medium at concentrations of 0, 12.5, 25, and 50 µg/mL. Nodule cultures were stained with 2% alizarin red S on day 15. The stained area was quantified by NIH Imaging. Data are means ± SE of three culture wells. Significantly different from culture without OFE at *p < 0.05, **p < 0.001.
